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M9480125.TXT
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1994-08-09
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Document 0125
DOCN M9480125
TI Projection structures of human immunodeficiency virus type 1 (HIV-1)
observed with high resolution electron cryo-microscopy.
DT 9410
AU Goto T; Ashina T; Fujiyoshi Y; Kume N; Yamagishi H; Nakai M; Department
of Microbiology, Osaka Medical College, Japan.
SO J Electron Microsc (Tokyo). 1994 Feb;43(1):16-9. Unique Identifier :
AIDSLINE MED/94292879
AB An electron microscopical study was made of human immunodeficiency virus
type 1 (HIV-1) in the unstained, frozen, hydrated state at liquid helium
temperature. The findings were compared with those obtained from
negative staining and ultrathin sectioning. Electron cryo-microscopy
confirms and extended the findings obtained by conventional methods. In
particular, the virus is shown to be globular, the lipid membrane is
clearly resolved as a bi-layer and the bi-layer was demonstrated to be
non-uniform in width. The projections studied in the bi-layer are
clearly observed to be knob-like and consist of a head, a stalk and a
base.
DE Freezing HIV-1/*ULTRASTRUCTURE Membranes/ULTRASTRUCTURE Microscopy,
Electron/*METHODS Support, Non-U.S. Gov't Viral Envelope
Proteins/*ULTRASTRUCTURE JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).